On day 21, the enzyme linked immunosorbent assay (ELISA) analysis was carried out on the serum sample. High density lipoprotein (HDL,Hengyuan), low density lipoprotein (LDL, Hengyuan), leptin (RD) and insulin (Alpco) were measured according to the manufacturers’ instruction. Three repeats were used and the microplate reader was used to test the signal (Thermo Fisher). Total RNA was isolated and purified using RNAiso Plus (TaKaRa) from abdominal fat (WAT, white adipose tissue) and liver. And cDNA was synthesized using the Transcriptor First Strand cDNA Synthesis Kit (Roche) in accordance with the manufacturer’s instructions. Primers information was shown in Table S1.